bs-2116R [Primary Antibody]
Rabbit  Anti-E Tag Polyclonal Antibody
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Host: Rabbit

Target Protein: E Tag

IR: Immunogen Range:GAPVPYPDPLEPR

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: N/A

Swiss Prot: N/A

Source: KLH conjugated E Tag peptide:GAPVPYPDPLEPR 

Purification: affinity purified by Protein A

Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background: E Tag is a commonly used epitope tag engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized. Because of the small size of the epitope, it is unlikely to alter the activity of the cloned sequence.

Size: 100ul

Concentration: 1mg/ml

Applications: WB=1:1000-5000,ELISA=1:5000-10000

Cross Reactive Species: Species independent

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: Lane1: E-Tagged Fusion Protein Overexpression E.coli Lysate at 2ug Lane2: E-Tagged Fusion Protein Overexpression E.coli Lysate at 2ug Primary: Anti- E tag (bs-2116R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kD Observed band size: 45 kD
 
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