bs-2122R [Primary Antibody]
Rabbit  Anti-GST tag Polyclonal Antibody
www.bioss.com.cn
sales@bioss.com.cn
techsupport@bioss.com.cn
400-901-9800
DATASHEET

Host: Rabbit

Target Protein: GST tag

IR: Immunogen Range:

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: N/A

Swiss Prot: N/A

Source: Recombinant full length GST tag protein: 

Purification: affinity purified by Protein A

Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background: GST is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.

Size: 100ul

Concentration: 1mg/ml

Applications: WB=1:20000-60000,ELISA=1:5000-10000

Cross Reactive Species: Species independent

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Transformed (GST-tag or EV) E. coli cells lysates were subjected to SDS-PAGE followed by WB with bs-2122R (Anti-GST) at dilution of 1:60,000 incubated at 4℃ overnight.
Sample: Lane 1: GST-Tagged Fusion Protein Overexpression E.coli Lysate Primary: Anti-GST tag (bs-2122R-HRP) at 1/1000 dilution Predicted band size: 41 kDa Observed band size: 45 kDa
Sample: GST-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41230P) at 4 ug Primary: Anti-GST Tag (bs-2122R) at 1/1000 ~ 1/10000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 41 kD
Sample: Lane 1: GST-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 2ug Lane 2: GST-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 4ug Primary: Anti-GST tag (bs-2122R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kD
Sample: Recombinant GST Protein Lysate at 40 ug Primary: Anti-GST tag (bs-2122R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 26 kD Observed band size: 27 kD
 
PRODUCT SPECIFIC PUBLICATIONS