bs-1644R [Primary Antibody]
Rabbit  Anti-phospho-EGFR (Tyr1068) Polyclonal Antibody
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Host: Rabbit

Target Protein: phospho-EGFR (Tyr1068)

IR: Immunogen Range:QR(p-Y)SS

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1956

Swiss Prot: P00533

Source: KLH conjugated Synthesised phosphopeptide derived from human EGFR around the phosphorylation site of Tyr1068:QR(p-Y)SS 

Purification: affinity purified by Protein A

Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background: The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

Size: 100ul

Concentration: 1mg/ml

Applications: IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=0.2μg /test,ICC=1:100,IF=1:100-500,ELISA=1:5000-10000

Cross Reactive Species: Human,Rat (predicted: Mouse,Dog)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p-EGFR (Tyr1068)) Polyclonal Antibody, Unconjugated (bs-1644R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (phospho-EGFR (Tyr1068)) polyclonal Antibody, Unconjugated (bs-1644R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control (blue line): A431 (blue). Primary Antibody (green line): Rabbit Anti- Phospho-EGFR (Tyr1068) antibody (bs-1644R) Dilution: 0.2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
 
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