bs-1329R [Primary Antibody]
Rabbit  Anti-ZO-1/TJP1 Polyclonal Antibody
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Host: Rabbit

Target Protein: ZO-1/TJP1

IR: Immunogen Range:1551-1702/1733

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7082

Swiss Prot: Q07157

Source: KLH conjugated synthetic peptide derived from human ZO-1:1551-1702/1733 

Purification: affinity purified by Protein A

Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background: This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family of proteins, and acts as a tight junction adaptor protein that also regulates adherens junctions. Tight junctions regulate the movement of ions and macromolecules between endothelial and epithelial cells. The multidomain structure of this scaffold protein, including a postsynaptic density 95/disc-large/zona occludens (PDZ) domain, a Src homology (SH3) domain, a guanylate kinase (GuK) domain and unique (U) motifs all help to co-ordinate binding of transmembrane proteins, cytosolic proteins, and F-actin, which are required for tight junction function. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017]

Size: 100ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1μg/Test,IF=1:100-500,ELISA=1:5000-10000

Cross Reactive Species: Human,Pig (predicted: Mouse,Rat,Rabbit,Cow,Chicken,Dog,GuineaPig)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: Lane 1: Huvec (Human) Cell Lysate at 30 ug Lane 2: A431 (Human) Cell Lysate at 30 ug Primary: Anti-ZO-1 (bs-1329R) at 1/1000 dilution Anti-beta-Actin (bs-0061R) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 220 kD Observed band size: 220 kD
Independently Validated Antibody, image provided by Science Direct, badge number 029577:Formalin-fixed and paraffin embedded human testis labeled with Anti-ZO-1 Polyclonal Antibody, Unconjugated (bs-1329R) at 1:250 followed by conjugation to the secondary antibody.
Black line : Positive blank control (293T); Negative blank control (HL60） Green line : Primary Antibody (Rabbit Anti-ZO-1 antibody (bs-11320R) ) Orange line：Isotype Control Antibody (Rabbit IgG) . Blue line : Secondary Antibody (Goat anti-rabbit IgG-AF647) 293T（Positive）and HL60（Negative control）cells (black) were fixed with 4% PFA for 10min at room temperature, permeabilized with PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ZO-1 Antibody(bs-1329R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Blank control: 293T Cells(blue). Primary Antibody: Rabbit Anti-ZO-1/AF647 Conjugated antibody (bs-1329R-AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions. Protocol The cells were washed twice with phosphate-buffered saline (PBS). The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the antibody (bs-1329R-AF647, 1μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed.
 
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